IN VIVO ANTI-PYRETIC AND ANTI-INFLAMMATORY ACTIVITY OF CHITOSAN-REDUCED SILVER NANOPARTICLES USING MICE MODELS

Abstract

From this research the synthesized chitosan reduced AgNPs were examined by spectroscopic methods, AgNPs are primarily spherical with some degree of agglomeration, according to SEM examination. The effective synthesis is confirmed by the homogeneous distribution of nanoparticles with diameters in the nanometer ranges was observed at 70-100 nm, the produced nanoparticles showed a moderately polydispersed system with a Z-average size of 394.1 nm. With an intensity-based mean size of 273.3 nm, the effective synthesis of AgNPs is evident from the presence of a significant silver (Ag) signal with a weight percentage of 46.59% and an atomic percentage of 12.51%.Furthermore, components that correlate to the chitosan capping and stabilizing agent utilized in the synthesis are seen, including carbon (C), nitrogen (N), and oxygen (O). Remaining ions from the reaction medium may be the cause of the potassium (K) presence. XRD analysis confirmed the amorphous nature of the chitosan matrix is shown by the wide diffraction peak about 23.99° (2θ) in the XRD study of chitosan-mediated AgNPs. The lack of high and sharp peaks indicates that the silver nanoparticles are stable and well-embedded in the chitosan polymer, potentially in a semi-crystalline or amorphous phase as a result of chitosan capping, nano-drugs was checked invio in mice model against anti-inflammatory and anti-pyretic Activity they showed active potential over a 3-hour period they are investigated which works in one hour against inflammation 33.35 % and 30.19 % against pyrexia.